Michael W. NestorSamson JacobBruce SunDeborah PrèAndrew A. SproulSeong Im HongChris WoodardMatthew ZimmerVorapin ChinchalongpornOttavio ArancioScott A. NoggleThe New York Stem Cell Foundation Research InstituteColumbia University in the City of New YorkHunter CollegeMahidol UniversityHussman Institute for Autism2018-11-232018-11-232015-01-01American Journal of Physiology - Cell Physiology. Vol.308, No.3 (2015), C209-C21915221563036361432-s2.0-84922262889https://repository.li.mahidol.ac.th/handle/20.500.14594/35626© 2015 the American Physiological Society. Production and isolation of forebrain interneuron progenitors are essential for understanding cortical development and developing cell-based therapies for developmental and neurodegenerative disorders. We demonstrate production of a population of putative calretinin-positive bipolar interneurons that express markers consistent with caudal ganglionic eminence identities. Using serum-free embryoid bodies (SFEBs) generated from human inducible pluripotent stem cells (iPSCs), we demonstrate that these interneuron progenitors exhibit morphological, immunocytochemical, and electrophysiological hallmarks of developing cortical interneurons. Finally, we develop a fluorescence-activated cell-sorting strategy to isolate interneuron progenitors from SFEBs to allow development of a purified population of these cells. Identification of this critical neuronal cell type within iPSC-derived SFEBs is an important and novel step in describing cortical development in this iPSC preparation.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1152/ajpcell.00263.2014