Nuanchawee WetprasitMontri ChulavatnatolMahidol University2018-07-042018-07-041997-06-01Biochemistry and Molecular Biology International. Vol.42, No.2 (1997), 399-408103997122-s2.0-0031401760https://repository.li.mahidol.ac.th/handle/20.500.14594/17893Sugar-lectin binding assay was developed as a simple method which employed direct coating of microtiter plate with galactose-binding lectins. Biotin-galactose conjugate was used to bind to the immobilized lectins. The bound conjugate was then detected using streptavidin-horseradish peroxidase. Using the assay in conjunction with various competing carbohydrates, jackfruit lectin from Artocarpus heterophyllus was found to be specific for α-anomer of galactoside with an aromatic residue.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyDetermination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plateArticleSCOPUS