Sumalee JindadamrongwechNoppawan TungbupphaSuporn ChuncharuneePunnee ButthepFaculty of Medicine, Ramathibodi Hospital, Mahidol UniversityMahidol University2018-09-242018-09-242010-04-01Hemoglobin. Vol.34, No.2 (2010), 161-1641532432X036302692-s2.0-77950482755https://repository.li.mahidol.ac.th/handle/20.500.14594/28743The S-window hemoglobin (Hb) variants revealed by high performance liquid chromatography (HPLC) were studied in 12 Thai individuals. The variants were identified, using DNA sequencing and multiplex amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), to be six cases of Hb Tak [β147 (AC)], and six cases of Hb Q-Thailand [α74(EF3)Asp→His], respectively. By using the Capillarys 2-capillary zone electrophoresis (CE), Hb Tak and Hb Q-Thailand co-migrated with Hb F in zone 7. This might pose a problem as the high Hb F conditions suggest a differential diagnosis. The S-window Hb variants are mostly Hb Tak and Hb Q-Thailand in the Thai population rather than Hb S [β6(A3)Glu→Val]. The definite identification of Hb variants detected by HPLC or capillary electrophoresis (CE) requires DNA analysis. © Informa UK, Ltd.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.3109/03630261003679631