Pranee InprakhonPabhangkon PanlawanThunyarat PongtharankulEmmanuelle MarieLars O. WiemannAlain DurandVolker SieberMahidol UniversityFraunhofer Institute for Interfacial Engineering and Biotechnology IGBCNRS Centre National de la Recherche ScientifiqueUniversité de Lorraine2018-11-092018-11-092014-01-01Colloids and Surfaces B: Biointerfaces. Vol.113, (2014), 254-26018734367092777652-s2.0-84885036026https://repository.li.mahidol.ac.th/handle/123456789/33457The preparation of polyester particles using enzyme-catalyzed (lipase from Candida antarctica B, CALB) ring-opening polymerization of e-caprolactone (e-CL) in aqueous dispersion was demonstrated for the first time. Immobilization of CALB enabled a significant increase of the number-average degree of polymerization of e-CL oligomers (up to 38) as compared to dissolved CALB (8 at the maximum). The nature and amount of lipase, as well as the nature of the support material were identified as key parameters controlling ring-opening polymerization of e-CL in aqueous dispersion. In addition, the involvement of solubilized monomers in polymerization elementary reactions was demonstrated and the consequences on oligomers average length were detailed. An overall mechanism of lipase-catalyzed e-CL polymerization in aqueous dispersion taking into account the colloidal nature of reaction medium was proposed on the basis of experimental results. © 2013 Elsevier B.V.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringChemistryPhysics and AstronomyArticleSCOPUS10.1016/j.colsurfb.2013.09.011