Sineewanlaya WichitAkanitt JittmittraphapKazuya I.P.J. HidariButsaya ThaisomboonsukSongsak PetmitrSukathida UbolChie AokiSaki ItonoriKoichi MoritaTakashi SuzukiYasuo SuzukiWipawee JampangernMahidol UniversityArmed Forces Research Institute of Medical Sciences, San FranciscoUniversity of ShizuokaShiga UniversityNagasaki UniversityChubu UniversityUniversity of Tokyo2018-05-032018-05-032011-02-01Microbiology and Immunology. Vol.55, No.2 (2011), 135-14013480421038556002-s2.0-79251506368https://repository.li.mahidol.ac.th/handle/20.500.14594/12087Dengue viruses infect cells by attaching to a surface receptor which remains unknown. The putative receptor molecules of dengue virus type 2 on the surface of mosquito (AP-61) and mammalian (LLC-MK2) cell lines were investigated. The immunochemical detection and structural analysis of carbohydrates demonstrated that the neutral glycosphingolipids, L-3 (GlcNAcβ1-3Manβ1-4Glcβ1-1'Cer) in AP-61 cells, and nLc 4 Cer (Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1'Cer) in LLC-MK2 cells were recognized by the virus. These findings strongly suggest that neutral glycosphingolipids share the key determinant for virus binding and that the β-GlcNAc residue may play an important role in dengue virus binding to the host cell surface. © 2011 The Societies and Blackwell Publishing Asia Pty Ltd.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1111/j.1348-0421.2010.00293.x