Kanyanan KritsiriwuthinanWarunee NgrenngarmlertSakone SunantarapornAnna JehmahChulalongkorn UniversityRangsit UniversityMahidol UniversityMaelan Hospital2020-06-022020-06-022020-01-01Journal of Parasitology Research. Vol.2020, (2020)20900031209000232-s2.0-85084078248https://repository.li.mahidol.ac.th/handle/20.500.14594/56218© 2020 Kanyanan Kritsiriwuthinan et al. Genetic diversity of Plasmodium parasite has significantly related to malaria control and vaccine development. The P. falciparum merozoite surface protein 1 (Pfmsp1) gene is a commonly used molecular marker to differentiate genetic diversity. This study is aimed at developing a nested PCR-Heteroduplex Mobility Assay (nPCR-HMA) for determination of the block 2 of the Pfmsp1 gene. The MAD20 family allele of P. falciparum was used as a control for optimization of the annealing and polyacrylamide gel electrophoresis conditions. In order to evaluate the developed nPCR-HMA, 8 clinical P. falciparum isolates were examined for allelic variants. The results revealed 9 allelic variants. Our study indicated that the successful nPCR-HMA with good precision and accuracy offers a more rapid, efficient, and cheap method for large-scale molecular epidemiological studies as compared to nucleotide sequencing.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1155/2020/9520326