Triwit RattanarojpongHao Ching WangChu Fang LoTimothy W. FlegelMahidol UniversityNational Taiwan University2018-08-242018-08-242007-10-01Proteomics. Vol.7, No.20 (2007), 3809-381416159861161598532-s2.0-35648986063https://repository.li.mahidol.ac.th/handle/20.500.14594/24108In this proteomic analysis of gills from yellow head virus (YHV)-infected Penaeus vannamei, we identified 13 spots with up-regulated protein expression levels and five spots with down-regulated levels. LC-nanoESI-MS/MS indicated that the up-regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up-regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras-like GTP binding protein. By contrast, expression levels were reduced for an SCP-calcium binding protein (SCP), actin-1, a valosin-containing protein, and Rab11. Time-course assays by real time RT-PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1002/pmic.200700202