Wongsurawat T.Jenjaroenpun P.Nookaew I.Mahidol University2023-06-182023-06-182022-01-01Methods in Molecular Biology Vol.2477 (2022) , 71-7710643745https://repository.li.mahidol.ac.th/handle/20.500.14594/83911Direct RNA sequencing (dRNA-seq) simultaneously enables the detection of RNA modifications and characterization of full-length transcripts. In principle, full-length native RNA molecule is translocated through the nanopore by a motor protein while a sensor measures ionic current shifts. Then, the current shifts are interpreted by an algorithm that turn out to RNA sequence. Currently, the standard protocol of dRNA-seq provided by Oxford Nanopore Technologies (ONT) allows to directly ligate and sequence only polyadenylated RNA (poly(A) RNA). Here, we describe a method of dRNA-seq that can be applied for both poly(A) RNA and non-poly(A) tailed-RNA.Biochemistry, Genetics and Molecular BiologyBook ChapterSCOPUS10.1007/978-1-0716-2257-5_52-s2.0-851299991971940602935524112