Piyarat ThaijaroenKorapin SrisiriRapee BoonplueangNathinee PanvisavasMahidol University2018-05-032018-05-032011-12-01Forensic Science International: Genetics Supplement Series. Vol.3, No.1 (2011)1875175X187517682-s2.0-82455164317https://repository.li.mahidol.ac.th/handle/20.500.14594/11411In this study, we utilized a technique called ligation-mediated rolling circle amplification (L-RCA) that combined padlock probe ligation and rolling circle amplification techniques to determine human sex. Amelogenin X and Y specific padlock probes were designed based on polymorphisms and the 6-bp indel of the amelogenin X and Y alleles. Lengths of DNA target for padlock probe detection were 40 and 33 bases of amelogenin X and Y alleles, respectively. Analysis of female DNA samples produced one L-RCA product from the AmelX padlock probe, whereas 2 different types of products were amplified from male DNA samples, each generated by AmelX and AmelY padlock probes. Utilization of padlock probes via L-RCA technique would provide an alternative tool for human sex determination in highly degraded DNA analysis in the future. © 2011 Elsevier Ireland Ltd.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1016/j.fsigss.2011.09.110