Visith ThongboonkerdMahidol University2018-06-112018-06-112012-08-01Proteomics - Clinical Applications. Vol.6, No.7-8 (2012), 340-34218628354186283462-s2.0-84865603091https://repository.li.mahidol.ac.th/handle/123456789/13647Variability is one of the most critical issues of concern in clinical proteomics. In this issue of Proteomics Clinical Applications, Yoshida et al. [Proteomics Clin. Appl. 2012, 6, 412-417] describe the effects of blood and number of washes on the human glomerular proteome isolated from the kidney by laser microdissection. The blood-derived proteins occupied almost 50% of all the identified proteins in the unwashed samples, whereas varying the number of washes (from 1-5) with PBS yielded only 43-80% of the proteins identified in each sample that were common in all samples. This urges caution to all proteomists to carefully consider sample preservation and preparation for tissue proteome analysis. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyNoteSCOPUS10.1002/prca.201270035