Kalyanee JirasripongpunNuananong JirakanjanakitPremwadee SakulsomLa Ongdao WongekalakParichat HongsprabhasSilpakorn UniversityMahidol UniversityKasetsart University2018-06-112018-06-112012-09-01International Journal of Food Science and Technology. Vol.47, No.9 (2012), 1970-197613652621095054232-s2.0-84865197176https://repository.li.mahidol.ac.th/handle/123456789/13407This study investigated the cytotoxicity of asiatic acid (AA) encapsulated in different protein-carbohydrate matrices in human colon adenocarcinoma SW620, human hepatoblastoma HepG2 and diploid monkey kidney cells (Vero). Mungbean protein concentrate (MPC) and mungbean protein hydrolysate (MPH) in lactose (L) or lactose-maltodextrin (LM) mixture, were used to fabricate matrices encapsulating AA during lyophilisation. The cytotoxicity of AA was in a dose-dependent manner for HepG2 and SW620 cells, but not for Vero cells. The 50% inhibitory concentrations (IC 50 ) of AA in HepG2 and SW620 were 120 and 130μm respectively. The IC 50 of AA encapsulated in MPH-L matrix in HepG2 was reduced from that of AA alone to 80μm (P < 0.05). Study of the anti-cancer activity of AA, in the absence and presence of food matrices, revealed that an MPH-L matrix led AA to cause more apoptosis deaths in HepG2 cells than either a MPC-L matrix or pure AA. © 2012 Institute of Food Science and Technology.Mahidol UniversityAgricultural and Biological SciencesEngineeringArticleSCOPUS10.1111/j.1365-2621.2012.03058.x