F. HaqueS. FissehaP. AthamanolapR. TowerJ. OrtegaC. DominguezT. MarucaD. TorpeyR. MyersP. LaksanalamaiMahidol UniversityLaboratories Administration2020-11-182020-11-182020-11-01Journal of Microbiological Methods. Vol.178, (2020)18728359016770122-s2.0-85092451474https://repository.li.mahidol.ac.th/handle/20.500.14594/59857© 2020 Elsevier B.V. Carbapenemase Inactivation Method (CIM) is a test to detect presence of the carbapenemase in Gram-negative bacteria. Determination of the carbapenemase production by inactivation of meropenem requires that a zone of control E. coli inhibition be measured approximately 6–24 h after plating. We have modified the CIM test by developing a rapid method which instead measures the growth of E. coli indicator strain ATCC 25922 using real-time PCR, referred to as a nucleic acid testing CIM (natCIM). Our natCIM, therefore reduces the detecting time from 6 to 24 h to approximately 4 h.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.mimet.2020.106072