Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand

Alan BrockmanSittaporn SinglamLucy PhiaphunSornchai LooareesuwanNicholas J. WhiteFrançois NostenShoklo Malaria Research UnitMahidol UniversityUniversity of QueenslandJohn Radcliffe HospitalMenzies School of Health Research2018-07-242018-07-242004-04-01Antimicrobial Agents and Chemotherapy. Vol.48, No.4 (2004), 1426-1429006648042-s2.0-1642543069https://repository.li.mahidol.ac.th/handle/123456789/21686A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/μl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.Mahidol UniversityMedicineField Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern ThailandArticleSCOPUS10.1128/AAC.48.4.1426-1429.2004