Napa TreetipsatitMontri ChulavatnatolMahidol University2018-10-122018-10-121982-01-01Experimental Cell Research. Vol.142, No.2 (1982), 495-499001448272-s2.0-0020448799https://repository.li.mahidol.ac.th/handle/123456789/30296Demembranated model of rat epididymal spermatozoa was employed to establish the conditions for the initiation of flagellar movement. Extensive initiation of the flagellar movement required 0.5 mM ATP, 1 μM cAMP and pH 7.9. The requirement for ATP was highly specific and can partially be replaced by 2′-deoxy-ATP only, but not by analogs of ATP or other nucleoside triphosphates. In contrast, the cAMP requirement was less specific and can partially be replaced by other cyclic nucleotides and cAMP-analogs except 2′-deoxy-cAMP and 2′,3′-cAMP. The data implied that the intracellular pH rise, not the cAMP increase, was the probable trigger for the initiation of sperm motility after ejaculation. During sperm maturation, the sperm motile apparatus appeared unchanged with respect to the above conditions of reactivation. © 1982.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/0014-4827(82)90397-4