Hevea latex lectin binding protein in C-serum as an anti-latex coagulating factor and its role in a proposed new model for latex coagulation

Rapepun WititsuwannakulPiyaporn PasitkulPattavuth JewtragoonDhirayos WititsuwannakulPrince of Songkla UniversityThaksin UniversitySongkhla Rubber Research Center, HatyaiMahidol University2018-07-122018-07-122008-02-01Phytochemistry. Vol.69, No.3 (2008), 656-662003194222-s2.0-38349189246https://repository.li.mahidol.ac.th/handle/20.500.14594/18760A distinct protein specifically recognized by its strong interaction with Hevea latex lectin (HLL) was detected in the aqueous C-serum fraction of centrifuged fresh latex. This C-serum lectin binding protein (CS-HLLBP) exhibited strong inhibition of HLL-induced hemagglutination. The CS-HLLBP was purified to homogeneity by a protocol that included ammonium sulfate fractionation, size exclusion and ion exchange chromatography. The purified CS-HLLBP had a specific HI titer of 0.23 μg ml-1. Its Mrs analyzed by SDS-PAGE was ca. 40 kDa and that by gel filtration was ca. 204 kDa. It has a pI value of 4.7, an optimum activity between pH 6 and10 and was heat stable up to 50 °C. The HI activity of CS-HLLBP was abolished upon treatment with chitinase. The CS-HLLBP inhibited HLL-induced rubber particle aggregation in a dose dependent manner. A highly positive correlation between CS-HLLBP activity and rubber yield per tapping was found. The correlations for fresh latex (r = 0.98, P < 0.01) and dry rubber (r = 0.95, P < 0.01) were both highly significant. This indicated that the CS-HLLBP might be used as a reliable marker for the mass screening of young seedlings to identify and select clones with potential to be superior producers of rubber. A latex anti-coagulating role of the CS-HLLBP is proposed. The findings described in this 3 paper series have been used to propose a new model of rubber latex coagulation that logically describes roles for the newly characterized latex lectin and the two lectin binding proteins. © 2007 Elsevier Ltd. All rights reserved.Mahidol UniversityAgricultural and Biological SciencesBiochemistry, Genetics and Molecular BiologyChemistryPharmacology, Toxicology and PharmaceuticsHevea latex lectin binding protein in C-serum as an anti-latex coagulating factor and its role in a proposed new model for latex coagulationArticleSCOPUS10.1016/j.phytochem.2007.09.021