K. PattanapanyasatV. PengruangrojanachaiC. ThepthaiS. SuwanagoolC. WasiMahidol University2018-02-272018-02-271994-12-01Asian Pacific Journal of Allergy and Immunology. Vol.12, No.2 (1994), 105-1090125877X2-s2.0-0028672504https://repository.li.mahidol.ac.th/handle/20.500.14594/9564A three-color flow cytometric determination of CD4 T-lymphocytes on whole blood specimens from AIDS patients which contain a high proportion of non-lymphocyte elements is described. Peripheral blood cells were stained by a three-color method using monoclonal antibodies conjugated respectively with fluorescein isothiocyanate (FIT)-CD3, phycoerythrin (PE)-CD4 and peridinin chlorophyll protein (PerCP)-CD45, CD45 stains all leukocytes with the highest fluorescence expression of CD45 antigen in lymphocytes. By combining light scatter with CD45 in the fluorescence 3 (FL3) channel, a light scattering window can be drawn to include almost all bright CD45 lymphocytes. This live gate of lymphocytes was then acquired and analysed simultaneously using other irrelevant two-color (FITC/PE) antibodies of CD3 and CD4 in the ATC and PE channels, respectively, This method is easy and straightforward, and gives successful analysis of CD4 T-lymphocytes in AIDS blood specimens contaminated with an unusually large number of non-lymphocytic cells.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS