Atiphon PhatthiyaSeiji TakahashiNopphakaew ChareonthiphakornTanetoshi KoyamaDhirayos WititsuwannakulRapepun WititsuwannakulPrince of Songkla UniversityTohoku UniversityMahidol UniversityWalailak University2018-08-242018-08-242007-04-01Plant Science. Vol.172, No.4 (2007), 824-831016894522-s2.0-33847284023https://repository.li.mahidol.ac.th/handle/20.500.14594/24018Plastoquinones play important roles as electron carriers in the light-dependent reactions of photosynthesis and also as a cofactor of phytoene desaturation in the synthesis of carotenoid. A plastoquinone-9 (PQ-9) was identified in Frey-Wyssling organelles of fresh rubber latex from Hevea brasiliensis. This indicates that a Hevea solanesyl diphosphate synthase (HbSDS) must be present for the synthesis of the C45prenyl side chain of the PQ-9 found in the rubber latex. Based on the sequence information of other trans-prenyl diphosphate synthases, the cDNA encoding HbSDS was cloned from a Hevea rubber latex cDNA library. Sequence analysis revealed the presence of an ORF consisting of 1254 bp capable of encoding a 46,095 Da polypeptide. The deduced amino acid sequence of the clone contained all conserved regions found in typical trans-prenyl chain elongating enzymes. This cDNA, expressed in Escherichia coli cells as an insoluble but Trx-His-tagged fusion protein, showed a distinct solanesyl diphosphate synthase activity in the presence of Triton X-100. The expression level of HbSDS mRNA in latex was found to be higher than those in leaves, stems and roots. The expressed HbSDS is suggested to be a specific enzyme in the Frey-Wyssling particles of rubber latex. © 2007 Elsevier Ireland Ltd. All rights reserved.Mahidol UniversityAgricultural and Biological SciencesBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.plantsci.2006.12.015