Thunyanaratada TangsriJaturong PratuangdejkulSuchitra ThongpraditchoteVeena SatitpatipanPongpan Siripongวีณา สาธิตปัตติพันธ์สุจิตรา ทองประดิษฐ์โชติจตุรงค์ ประเทืองเดชกุลMahidol University. Faculty of Pharmacy. Department of MicrobiologyMahidol University. Faculty of Pharmacy. Department of PhysiologyMahidol University. Faculty of Pharmacy. Department of PharmacognosyDepartment of Public Health and Human Services. National Cancer Institute2020-01-282020-01-282020-01-282019https://repository.li.mahidol.ac.th/handle/20.500.14594/52391The 1st Pharmaceutical Sciences Asia Conference 2019 Theme : Pharmaceutical Sciences toward Health Innovation in the Disruptive Era. Bangkok Midtown Hotel, Thailand. August 22, 2019, page 53-58Suregada multiflora A. Juss. is frequently used as one of herbal medicine in Thai traditional recipes for treating dermatological diseases; i.e. Yaw disease, leprosy, eczema, psoriasis, and skin infection diseases. This research aimed to study antimicrobial activities of S. multiflora extracts which were carried out by serial manner of extraction (hexane, dichloromethane, and 95% ethanol) and the traditional extraction (decoction and maceration with aqueous and 95% ethanol, respectively). The Minimal Inhibitory Concentration (MIC) were determined by resazurin-based broth microdilution assay. The Minimal Bactericidal Concentration (MBC) were subsequently determined using drop-plate method. For Gram– positive bacteria and fungi, hexane extract of S. multiflora exhibited antimicrobial activity against Staphylococus epidermidis DMST 15471, Staphylococcus aureus ATCC 25923, Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300, Cutibacterium acnes DMST 14916, and Candida albicans DMST 5815 with the MIC values of 0.1563, 0.625, 0.625, 0.625, and 1.25 mg/mL, respectively. While, the MBC values of S. multiflora hexane crude extract were 0.3125, 2.5, and 5 mg/mL for S. epidermidis, C. acnes, and C. albicans, respectively. For S. multiflora dichloromethane crude extract, the MIC values of 0.0781, 0.625, 0.625, 1.25 and 2.5 mg/mL were respectively found for S. epidermidis, S. aureus, C. acnes, C. albicans, and MRSA, however, its MBC values were detected only in S. epidermidis and C. acnes with 0.625 and 1.25 mg/mL, respectively. From this study, the extracts of S. multiflora, revealed the in vitro potential antimicrobial activities against some skin pathogens which supports Thai traditional remedies use of S. multiflora for treatment of skin disease.engMahidol UniversitySuregada multifloraKhun-Thong PrayabaatAntimicrobial activityProceeding ArticleFaculty of Pharmacy Mahidol University