Fumiko NishidaMika NishimuraKen Ichi HaradaMakoto SuzukiVithaya MeevootisomTimothy W. FlegelYodhathai ThebtaranonthSuthum IntararuangsornMeijo UniversityMahidol University2018-02-272018-02-271994-04-01Journal of Chromatography A. Vol.664, No.2 (1994), 195-202002196732-s2.0-0028344959https://repository.li.mahidol.ac.th/handle/20.500.14594/9514The glycosidic antibiotics of the glykenin (GK) family produced by Basidiomycetes sp. were separated into nine components (GK-I-VII and DG) by normal-phase chromatography. It was found that these components differ in the number and location of the acetyl groups in the sugar moiety. Each component (GK-I-VII and DG) was further separated into three isomers (A, B and C), which possess different aglycones, by reversed-phase chromatography on an ODS column with methanol-acetonitrile as eluent. The best composition of the eluent was found to be methanol-acetonitrile-1% trifluoroacetic acid (4:3.5:2.5). The profile analysis of GK-III-VII and DG was also carried out using a modified mobile phase. The combination of normal- and reversed-phase chromatography separated all components of the GK mixture except GK-I and II. The relationship between structure and separation behaviour of GK is discussed. © 1994.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryArticleSCOPUS10.1016/0021-9673(94)87008-X