Chonprakun ThagunJiraporn SrisalaKallaya SritunyalucksanaJarunya NarangajavanaPunchapat SojikulMahidol UniversityCenter of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE)Thailand National Center for Genetic Engineering and Biotechnology2018-06-112018-06-112012-09-15Journal of Biotechnology. Vol.161, No.1 (2012), 60-6718734863016816562-s2.0-84864101662https://repository.li.mahidol.ac.th/handle/123456789/13612White spot syndrome virus is currently the leading cause of production losses in the shrimp industry. Penaeus monodon Rab7 protein has been recognized as a viral-binding protein with an efficient protective effect against white spot syndrome infection. Plant-derived recombinant PmRab7 might serve as an alternative source for in-feed vaccination, considering the remarkable abilities of plant expression systems. PmRab7 was introduced into the Arabidopsis thaliana T87 genome. Arabidopsis-derived recombinant PmRab7 showed high binding activity against white spot syndrome virus and a viral envelope, VP28. The growth profile of Arabidopsis suspension culture expressing PmRab7 (ECR21# 35) resembled that of its counterpart. PmRab7 expression in ECR21# 35 reached its maximum level at 5mgg -1 dry weight in 12 days, which was higher than those previously reported in Escherichia coli and in Pichia. Co-injection of white spot syndrome virus and Arabidopsis crude extract containing PmRab7 in Litopenaeus vannamei showed an 87% increase in shrimp survival rate at 5 day after injection. In this study, we propose an alternative PmRab7 source with higher production yield, and cheaper culture media costs, that might serve the industry's need for an in-feed supplement against white spot syndrome infection. © 2012 Elsevier B.V.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.1016/j.jbiotec.2012.05.007