Pitchanee JariyapongCharoonroj ChotwiwatthanakunMonsicha SomritSarawut JitrapakdeeLi XingHolland R. ChengWattana WeerachatyanukulMahidol UniversityWalailak UniversityUniversity of California, Davis2018-11-092018-11-092014-01-22Virus Research. Vol.179, No.1 (2014), 140-14618727492016817022-s2.0-84892790682https://repository.li.mahidol.ac.th/handle/20.500.14594/33313Virus-like particles (VLPs) are potential candidates in developing biological containers for packaging therapeutic or biologically active agents. Here, we expressed Macrobrachium rosenbergii nodavirus (MrNv) capsid protein (encoding amino acids M1-N371 with 6 histidine residuals) in an Escherichia coli BL21(DE3). These easily purified capsid protein self-assembled into VLPs, and disassembly/reassembly could be controlled in a calcium-dependent manner. Physically, MrNv VLPs resisted to digestive enzymes, a property that should be advantageous for protection of active compounds against harsh conditions. We also proved that MrNv VLPs were capable of encapsulating plasmid DNA in the range of 0.035-0.042. mol ratio (DNA/protein) or 2-3 plasmids/VLP (assuming that MrNV VLPs is T= 1, i made up of 60 capsid monomers). These VLPs interacted with cultured insect cells and delivered loaded plasmid DNA into the cells as shown by green fluorescent protein (GFP) reporter. With many advantageous properties including self-encapsulation, MrNv VLPs are good candidates for delivery of therapeutic agents. © 2013 Elsevier B.V.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.virusres.2013.10.021