Sasiporn PanasophonkulSomjai ApisawetakanScott F. CumminsPatrick S. YorkBernard M. DegnanPeter J. HannaPorncharn SaitongdeePrasert SobhonPrapee SretarugsaMahidol UniversityDeakin UniversityUniversity of Queensland2018-09-132018-09-132009-05-01Histochemistry and Cell Biology. Vol.131, No.5 (2009), 629-642094861432-s2.0-64049094651https://repository.li.mahidol.ac.th/handle/123456789/27236In molluscs, the neurotransmitter serotonin (5-HT) has been linked to a variety of biological roles including gamete maturation and spawning. The possible involvement of 5-HT in abalone gamete release was demonstrated by a dose-dependent increase in Haliotis rubra gonad contractile bioactivity following 5-HT stimulation. Physiological functions associated with 5-HT, are mediated through binding to 5-HT receptors. A cDNA encoding a putative 5-HT receptor consisting of 359 amino acids was isolated from the tropical abalone H. asinina, termed 5-HT1 ha. The 5-HT1 hashares G-protein-coupled receptor motifs with metazoan 5-HT receptors, including predicted transmembrane domains, active sites for protein kinase action, and N-linked glycosylation sites. However, the third intracellular loop of 5-HT1 hais relatively short, and only six transmembrane domains are predicted, implying a truncated receptor. Phylogenetic analysis with known 5-HT receptor genes suggests that 5-HT1 habelongs to the type 1 5-HT receptor family. Expression analysis by RT-PCR showed that 5-HT1 hamRNA was present in all tissues examined, including the neural ganglia and gonad tissues. Immunocytochemistry revealed the presence of 5-HT1 haspecifically within the soma of neuronal cells located in the outer cortex of both cerebral and pleuropedal ganglia. In ovarian and testicular tissues, 5-HT1 haimmunoreactivity was observed in epithelial cells of the outer capsule and connective tissue of the trabeculae to which the gamete follicles adhere. Whether this receptor transcript is translated to a functional protein needs to be verified, but if so, it could play a role in reproduction. © 2009 Springer-Verlag.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyHealth ProfessionsMedicineArticleSCOPUS10.1007/s00418-009-0555-7