Intatham S.Taychaworaditsakul W.Khonsung P.Chansakaow S.Jaijoy K.Lertprasertsuke N.Soonthornchareonnon N.Sireeratawong S.Mahidol University2025-01-032025-01-032024-12-01Biology Vol.13 No.12 (2024)https://repository.li.mahidol.ac.th/handle/20.500.14594/102598Maha Pigut Triphala is a herbal formula composed of dry fruits of three plants, namely, Terminalia bellirica, Terminalia chebula, and Emblica officinalis also known as Phyllanthus emblica, which are used in different proportions in traditional Thai medicine. This formula has long been used to treat colds, relieve coughing, and alleviate phlegm. Nevertheless, there have been no studies on the safety of Maha Pigut Triphala in the ratio of 2:1:3. Thereby, this study aimed to investigate the acute and chronic oral toxicity of Maha Pigut Triphala in the ratio of 2:1:3 in Sprague-Dawley rats. The results showed that the rats that were given a single dose of Maha Pigut Triphala and those that received Maha Pigut Triphala once daily for 270 days in acute and chronic oral toxicity studies, respectively, did not exhibit any abnormal behaviors or mortality. There were only minor changes in body weight, organ weight, hematological and blood chemistry values. However, gross pathological and histopathological studies revealed no abnormalities in the internal organs. Therefore, it can be concluded that Maha Pigut Triphala in the ratio of 2:1:3 causes neither acute nor chronic oral toxicity in rats. Additionally, the present study provides safety information on Maha Pigut Triphala in animals that could help guide future studies in humans.Biochemistry, Genetics and Molecular BiologyAgricultural and Biological SciencesImmunology and MicrobiologyArticleSCOPUS10.3390/biology131210052-s2.0-8521322714620797737