Chareerut PhruksaniyomPermphan DharmasarojaSurapol IssaragrisilMahidol University. Faculty of Science. Department of Anatomy2017-08-092017-08-092017-08-092013Experimental Hematology & Oncology. Vol. 2, (2013), 9https://repository.li.mahidol.ac.th/handle/20.500.14594/2730Less is known about the non-mesenchymal mononuclear cell fraction of human bone marrow on functional adaptation of neuroblastoma cells. Using immunocytochemistry, we showed that bone-marrow mononuclear cell (BMMC)-conditioned medium can induce tyrosine hydroxylase expression in neuroblastoma cells, which is similar to the effect of retinoic acid. Using quantitative RT-PCR, we showed that NGF, CNTF, and BDNF mRNAs were detected in unfractionated BMMC populations from all human donors at different expression levels. Our results suggest that cells of the non-mesenchymal mononuclear cell fraction can induce functional adaptation of neuroblastoma cells, probably via their secreted trophic factors.engMahidol UniversityOpen Access articleBone-marrow mononuclear cellsNeuroblastoma cellsTyrosine hydroxylaseTrophic factorsNeuronal differentiationArticleBioMed Central10.1186/2162-3619-2-9