Arunee AhantarigPremnika MalaisriSupanee HirunkanokpunChalao SumrandeeWachareeporn TrinachartvanitVisut BaimaiMahidol UniversityLSU School of Veterinary Medicine2018-05-032018-05-032011-05-01Current Microbiology. Vol.62, No.5 (2011), 1496-150214320991034386512-s2.0-79954419328https://repository.li.mahidol.ac.th/handle/20.500.14594/12051In this study, we identified two Haemaphysalis species present at the Khao Yai National Park in Thailand and investigated the presence of rickettsia in these ticks. A total of 166 Haemaphysalis specimens were collected randomly under leaves along visitor paths at five locations in the park. Male and female adults of two different Haemaphysalis species, H. shimoga and H. lagrangei, were identified. Polymerase chain reaction (PCR) analysis revealed Rickettsia bacteria in these two Haemaphysalis species; this study represents the first time such presence has been reported in Thailand. The infection rates of Rickettsia were in both H. shimoga (7.41%) and H. lagrangei (10.17%) at these locations in addition to two pools of Haemahysalis nymphs (28.57%). Furthermore, 25.93% of H. shimoga showed positive results that matched Haemaphysalis longicornis symbionts (92% sequence identity) and the Coxeilla burnetti 16S ribosomal RNA gene (90% sequence identity). We propose that this is a novel H. shimoga symbiont bacterium in Thailand and might be a novel Coxeilla-like agent or Coxeilla sp. found in H. shimoga. In contrast, we did not observe any Wolbachia bacteria, which also belong to the order Rickettsiales, in the same group of Haemaphysalis ticks. Furthermore, PCR was used to detect three other genera of bacteria, Anaplasma, Ehrlichia and Borrelia, none of which were identified in the Haemaphysalis ticks studied. © 2011 Springer Science+Business Media, LLC.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1007/s00284-011-9887-3