Busaba PanyarachunArin NgamniyomPrasert SobhonPanat AnuracpreedaMahidol University. Kanchanaburi Campus. Agricultural ScienceMahidol University.Faculty of Science. Department of AnatomyMahidol University.Division of Agricultural Science.2015-08-042017-03-142015-08-042017-03-1420132013Journal of Veterinary Science. Vol.14, No.4 (Dec 2013), 425-32https://repository.li.mahidol.ac.th/handle/20.500.14594/1376In the present study, we evaluated the histological morphology of the adult Paramphistomum (P.) gracile. Adult flukes with bodies 5∼15 mm in length and 2∼7 mm in width were subjected to histological analysis. Longitudinal and transversal serial-sections were stained with hematoxylin and eosin, and examined. The body surface and longitudinal section of P. gracile were also assessed using scanning electron microscopy. In this species, the anterior sucker and posterior sucker (acetabulum) were present on an anterior and posterior part of the body, respectively. The major folds were located in the areas of the anterior sucker, genital canal, and posterior sucker. The fluke membrane was spineless at the tegument surface and in the tegument tissue. Histological data showed structural-systematic characteristics of the digestive tract, reproductive tract, excretory tract, copulatory organs, connective tissues, and muscle tissues. We attempted to elucidate the histological characteristics of P. gracile that might increase the knowledge and understanding of rumen fluke morphology.enghistologylight microscopymorphologyParamphistomum gracilescanning electron microscopyOpen Access articleArticleThe Korean Society of Veterinary Science.http://dx.doi.org/10.4142/jvs.2013.14.4.425