M. D. SmithB. J. AngusV. WuthiekanunN. J. WhiteMahidol UniversityNuffield Department of Clinical MedicineMusgrove Park Hospital2018-07-042018-07-041997-10-01Infection and Immunity. Vol.65, No.10 (1997), 4319-4321001995672-s2.0-0030843506https://repository.li.mahidol.ac.th/handle/20.500.14594/17960Two distinct types of Burkholderia pseudomallei, differentiated by the ability to assimilate L-arabinose but with similar morphologies and antigenicities, can be isolated from soil in Thailand. Approximately 25% of soil isolates from northeast Thailand were arabinose assimilators (Ara+), but in 1,200 sequentially studied patients, only arabinose 'nonassimilators' (Ara-) caused melioidosis (P < 0.0001). In a murine model, there was a striking difference in virulence between Ara-and Ara+B. pseudomallei. The mean (standard deviation) 50% lethal dose (LD50) inoculum for Ara-isolates was 182 (111) CFU/mouse compared with approximately 109CFU/mouse for Ara+soil isolates. There was no significant difference between the LD50s for clinical and soil Ara-isolates. All attempts to convert the biochemical phenotype by selective culture failed, which suggests that the biotype is stable.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS