Mintra KaewkittikhunNittaya BoonmuenPakpoom KheolamaiSirikul ManochantrChairat TantrawatpanNareerat SutjaritDuangrat TantikanlayapornRamathibodi HospitalFaculty of Medicine, Thammasat UniversityMahidol UniversityThammasat University2022-08-042022-08-042021-08-18Journal of Agricultural and Food Chemistry. Vol.69, No.32 (2021), 9259-926915205118002185612-s2.0-85114030187https://repository.li.mahidol.ac.th/handle/20.500.14594/75601Obesity has become a major public health concern; so, a strategy to prevent or reduce obesity is a priority. The inhibition of lipid droplet accumulation and adipogenesis process provides a target for the treatment of obesity. Herein, the effect of andrographolide (AP) on lipid accumulation in adipocytes derived from human bone marrow mesenchymal stem cells (hBM-MSCs) was examined. AP at concentrations of 1, 2.5, 5, and 10 μM reduced lipid droplet accumulation in the adipocytes by suppressing the adipogenic differentiation of hBM-MSCs. Concurrently, the expressions of adipogenic marker genes and the level of adipokines secreted by adipocytes were suppressed. Gene screening analysis showed a negative regulation of genes involved in the adipogenesis process. In conclusion, we demonstrated for the first time an antilipid accumulation in adipocytes from hBM-MSCs by AP. The compound may potentially be a novel therapeutic agent for the treatment of obesity as well as obesity-related diseases.Mahidol UniversityAgricultural and Biological SciencesChemistryArticleSCOPUS10.1021/acs.jafc.1c02724