A. NamsaiS. LouisirirotchanakulN. WongchindaU. SiripanyaphinyoP. VirulhakulP. PuthavathanaK. S. MyintM. GannarongR. IttapongMahidol UniversityFishery Technological Development DivisionResearch Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI)Central GovernmentArmed Forces Research Institute of Medical Sciences, ThailandFish Inspection and Quality Control Division2018-05-032018-05-032011-12-01Letters in Applied Microbiology. Vol.53, No.6 (2011), 608-6131472765X026682542-s2.0-81255185092https://repository.li.mahidol.ac.th/handle/20.500.14594/11958Aims: To survey for hepatitis A virus (HAV) and hepatitis E virus (HEV) contamination in edible bivalve shellfish. Methods and Results: A total of 213 shellfish (52 oysters, 69 cockles and 92 mussels) collected from a culture farm and two retailed markets were investigated for HAV and HEV contamination by reverse transcription-polymerase chain reaction (RT-PCR) assay using HA2-HA1 (capsid region) and HE366-HE363 (ORF2/3 overlapping region) primers, respectively. It was found that 3·8% of the shellfish and 2·9 and 6·5% of the cockle and mussel, respectively, showed positive for HAV detection. Nucleotide sequencing of all the 8 HAV-positive shellfish revealed 97-100% similarity to HAV subgenotype IA. Interestingly, viruses were found more frequently in the gills than in digestive tissue (4·5%vs 0·5%, P=0·045). All the shellfish were negative for HEV. Conclusion: Significant contamination of HAV in edible bivalve shellfish was observed. Beside digestive tissue, gills are one of the important samples for viral genome detection. Significance and Impact of the study: HAV-contaminated shellfish can play a role as reservoirs and/or vehicles in faecal-oral transmission in Thailand, and further monitoring of such a contamination is required. © 2011 The Society for Applied Microbiology.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1111/j.1472-765X.2011.03152.x