Parvapan BhattarakosolChalobon YoosookPornchai MatangkasombutMahidol University2018-08-102018-08-101991-01-01MICROBIOLOGY and IMMUNOLOGY. Vol.35, No.7 (1991), 525-53313480421038556002-s2.0-0025745229https://repository.li.mahidol.ac.th/handle/20.500.14594/22045Intratypic variation among 94 isolates of herpes simplex virus type 2 (HSV-2) was investigated using 4 different monoclonal antibodies (MAbs). By neutralization test, these MAbs appeared to be directed to at least 2 distinct epitopes on the viral glycoprotein D (gD), i.e., 6G6•G9and 6E8•F11which did not require complement (C-MAb) and gD-105 and gD-110 whose neutralizing activities could be enhanced by complement (C+MAb). The C-MAb pairs each separately could detect significant intratypic variations among the isolates. Whether these variations also existed in the gD epitope(s) recognized by C+MAbs remains to be elucidated. The results suggested that intratypic variation occurred on at least one of the neutralizable (thus related to protective immunity) epitopes on gD of HSV-2. © 1991, Center For Academic Publications Japan. All rights reserved.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1111/j.1348-0421.1991.tb01583.x