Charoenpanyakul R.Kittichai V.Eiamsamang S.Sriwichai P.Pinetsuksai N.Naing K.M.Tongloy T.Boonsang S.Chuwongin S.Mahidol University2024-11-222024-11-222024-12-01Scientific Reports Vol.14 No.1 (2024)https://repository.li.mahidol.ac.th/handle/20.500.14594/102117Traditional mosquito identification methods, relied on microscopic observation and morphological characteristics, often require significant expertise and experience, which can limit their effectiveness. This study introduces a self-supervised learning-based image classification model using the Bootstrap Your Own Latent (BYOL) algorithm, designed to enhance mosquito species identification efficiently. The BYOL algorithm offers a key advantage by eliminating the need for labeled data during pretraining, as it autonomously learns important features. During fine-tuning, the model requires only a small fraction of labeled data to achieve accurate results. Our approach demonstrates impressive performance, achieving over 96.77% accuracy in mosquito image analysis, with minimized both false positives and false negatives. Additionally, the model’s overall accuracy, measured by the area under the ROC curve, surpasses 99.55%, highlighting its robustness and reliability. A notable finding is that fine-tuning with just 10% of labeled data produces results comparable to using the full dataset. This is particularly valuable for resource-limited settings with limited access to advanced equipment and expertise. Our model provides a practical solution for mosquito identification, overcoming the challenges of traditional microscopic methods, such as the time-consuming process and reliance on specialized knowledge in healthcare services. Overall, this model supports personnel in resource-constrained environments by facilitating mosquito vector density analysis and paving the way for future mosquito species identification methodologies.MultidisciplinaryArticleSCOPUS10.1038/s41598-024-78260-22-s2.0-852091303382045232239511347