Piyapong HadpanusPermkun PermsirivisarnSitthiruk RoytrakulSumalee TungpradabkulMahidol UniversityThailand National Center for Genetic Engineering and Biotechnology2020-01-272020-01-272019-04-01Journal of Microbiological Methods. Vol.159, (2019), 26-3318728359016770122-s2.0-85061989437https://repository.li.mahidol.ac.th/handle/20.500.14594/50210© 2019 Elsevier B.V. A serious human infectious disease called Melioidosis is a result of Burkholderia pseudomallei infection. Treatment for infected individuals is difficult due to a wide range of ineffective antibiotics including a high level of antibiotic tolerance which has been known to be caused by biofilm production. However, biofilm forming processes of this bacterium are not well documented despite multiple-methodologies being applied. In this study, we utilized a proteomics strategy called whole cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (whole cell MALDI-TOF MS) to discover a potential biomarker relating biofilm forming in B. pseudomallei. The results presented a novel specific type of enzyme amylo-alpha-1, 6-glucosidase, which was demonstrated by a higher level of gene expression during the biofilm development. Our results also suggested a list of candidate markers that might be involved in this scenario. Eventually, this knowledge may expand valuable data to the biofilm study that may increase effective treatments for people infected with B. pseudomallei and possibly other antibiotic tolerant bacteria.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.mimet.2019.02.011