Ruangrat BuddhirongawatrSiriporn TungsudjaiKridsada ChaichouneCharoonluk SangloungNitipan TantawiwattananonRassameepen PhonaknguenYaowalark SukthanaMahidol University2018-08-202018-08-202006-12-01The Southeast Asian journal of tropical medicine and public health. Vol.37 Suppl 3, (2006), 15-17012515622-s2.0-34447530781https://repository.li.mahidol.ac.th/handle/20.500.14594/23418Toxoplasma gondii can infect all species of warm-blooded animals, including humans, and causes serious diseases in immunocompromized hosts. Live tachyzoites derived from serial passage in HeLa culture were used in the Sabin-Feldman dye test for detection of Toxoplasma gondii antibody in serum samples of 21 captive wild felids including one fishing cat (Prion nailurus viverrina), one leopard (Panthera pardus), two flat-headed cats (Prion nailurus planiceps), 6 tigers (Panthera tigris), two leopard cats (Felis bengalensis), two clouded leopards (Felis nebulosa), 3 pumas (Puma concolor), and 4 jungle cats (Felis chaus). Antibodies to Toxoplasma gondii were founded in 9 of 21 felids (42.8%). This study revealed that cell culture-derived tachyzoites can be used successfully as a source of live organisms in a gold standard Sabin-Feldman dye test, which is simpler, cheaper and less ethically sensitive than in vivo inoculation.Mahidol UniversityMedicineArticleSCOPUS