Supannee LeethochavalitKashane ChalermwatE. Suchart UpathamKwang Sik ChoiPichan SawangwongMaleeya KruatrachueBurapha UniversityJeju National UniversityMahidol University2018-07-242018-07-242004-08-09Diseases of Aquatic Organisms. Vol.60, No.2 (2004), 165-171017751032-s2.0-9944237390https://repository.li.mahidol.ac.th/handle/123456789/21071The undulated surf clam Paphia undulata supports Thailand's largest shellfishery in the Gulf of Thailand, with landings in 1999 recorded at 70 000 t (metric tonnes) yr-1. We report, for the first time, the prevalence of Perkinsus sp. in clams in the Gulf. A monthly survey from January to December 2001 utilizing the fluid thioglycollate medium (FTM) method showed that average monthly prevalence was 84.7% (n = 360). The monthly percentage of infected clams was generally 100%, with low prevalence in May (66.7%) and no infection in September. The monthly mean infection intensity in terms of Perkinsus sp. cells g-1tissue varied from 0 in September to 187 759 ± 18 970 (x ± SE) in October. No obvious annual variation in intensity and prevalence was observed. Prezoosporangia that developed in FTM were 25 to 75 μm in diameter. A few days after incubation in aerated seawater, the prezoosporangia underwent successive binary cell division and formed motile zoospores (2 to 5 μm long). The zoospores were released into the seawater through a discharge tube formed during the 2- and 4-cell stages. Serial semi-thin sections (1 to 4 μm thickness) of clam tissue (n = 120 clams) showed developing trophozoites 3 to 6 μm in diameter within gills, connective tissue, gonads and, especially, the digestive glands. Microscopic features of different life stages indicated that Perkinsus sp. in Thailand closely resembled P. olseni (= P. atlanticus) reported in Australia, New Zealand, Korea, Japan, Spain and Portugal.Mahidol UniversityAgricultural and Biological SciencesArticleSCOPUS10.3354/dao060165