Naeem AkbarSidra KanwalMuhammad Shafiq AhmedSimab KanwalMuhammad Ashfaq JamilSabastine EzugwuMuhammad SaleemSaid Nasir KhisroMuhammad JavedNasar AhmedAhsan EjazMirpur University of Science and TechnologyUniversity of Azad Jammu and KashmirWestern UniversityInstitute of Molecular Biosciences, Mahidol UniversityUniversity of Kotli Azad Jammu and Kashmir2022-08-042022-08-042021-01-01Brazilian Archives of Biology and Technology. Vol.64, (2021)16784324151689132-s2.0-85124007623https://repository.li.mahidol.ac.th/handle/123456789/79394There is a great interest to use carbon-based material like graphene and graphene oxide in biomedical applications due to its flexibility to be functionalized with bio-active molecules. Herein, graphene and graphene-based nanocomposites were biosynthesized by liquid-phase exfoliation of graphite using aqueous extract of Parthenium hysterophorous (P-H) as a surfactant. A set of five thin film samples of graphene was prepared from graphene suspension by vacuum filtration method. Samples were characterized by UV-vis spectroscopy, Raman spectroscopy, SEM, and XRD, which revealed successful synthesis of graphene. Graphene/P-H(G/P-H) nanocomposites comprising varied ratios of graphene and P-H were prepared and their antibacterial activity was investigated by agar well diffusion method. The experimental results indicated that G/P-H nanocomposite have higher antibacterial activity than graphene alone, and bioactivity of G/P-H nanocomposite was found to be controlled by the fraction of graphene in the composite.Mahidol UniversityMultidisciplinaryArticleSCOPUS10.1590/1678-4324-2021210180