Chonlakan SupasuteekulNarawat NuamnaichatiSupachoke MangmoolKittisak LikhitwitayawuidParkpoom TengamnuayWaraporn PutalunBoonchoo SritularakChulalongkorn UniversityMahidol UniversityKhon Kaen University2018-12-212019-03-142018-12-212019-03-142017-01-01Natural Product Communications. Vol.12, No.11 (2017), 1691-1694155594751934578X2-s2.0-85035243322https://repository.li.mahidol.ac.th/handle/20.500.14594/41607The leaves of Aquilaria crassna Pierre ex Lecomte (Thymelaeaceae) have been used in the traditional medicine and consumed as a health-promoting tea in several Asian countries. This study investigated the antioxidant activity of three major bioactive constituents of A. crassna leaves, genkwanin 5-O-β- primeveroside (1), iriflophenone 3,5-C-β-D-diglucoside (2) and iriflophenone 3-C-β-D-glucoside (3) using DPPH and superoxide scavenging assays, as well as their effects on induction of mRNA and protein expressions of antioxidant enzymes in HEK-293 cells. The benzophenone glycoside 3 exhibited significant scavenging activity against both types of free radicals and increased the mRNA expression of antioxidant enzymes such as glutathione peroxidase-1, glutathione reductase and heme oxygenase-1 in HEK-293 cells. In addition, all three compounds also increased the protein expression of glutathione peroxidase-1. These findings revealed the effects of these compounds at cellular level, and might be useful for the development of A. crassna leaves as supplements for prevention of oxidative stress.Mahidol UniversityAgricultural and Biological SciencesArticleSCOPUS