Sakan WarinhomhounChawanphat MuangnoiVisarut BuranasudjaWanwimon MekboonsonglarpPornchai RojsitthisakKittisak LikhitwitayawuidBoonchoo SritularakChulalongkorn UniversityMahidol University2022-08-042022-08-042021-02-01Antioxidants. Vol.10, No.2 (2021), 1-16207639212-s2.0-85100501287https://repository.li.mahidol.ac.th/handle/20.500.14594/76291Five compounds including a new bisbibenzyl named dendropachol (1) and four known compounds (2–5) comprising 4,5-dihydroxy-2,3-dimethoxy-9,10-dihydrophenanthrene (2), gigantol (3), moscatilin (4) and 4,5,4′-trihydroxy-3,3′-dimethoxybibenzyl (5) were isolated from a methanolic extract of Dendrobium pachyglossum (Orchidaceae). The chemical structures of the isolated compounds were characterized by spectroscopic methods. Dendropachol (1) was investigated for its protective effects on hydrogen peroxide (H2O2 )-induced oxidative stress in HaCaT keratinocytes. Compound 1 showed strong free radical scavenging compared to the positive control. For the cytoprotective effect, compound 1 increased the activities of GPx and CAT and the level of GSH but reduced intracellular reactive oxygen species (ROS) generation and accumulation. In addition, compound 1 significantly diminished the expression of p53, Bax, and cytochrome C proteins, decreased the activities of caspase-3 and caspase-9, and increased Bcl-2 protein. The results suggested that compound 1 exhibited antioxidant activities and protective effects in keratinocytes against oxidative stress induced by H2O2 .Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.3390/antiox10020252