Lukana NgiwsaraChantragan SrisomsapPranee WinichagoonSuthat FucharoenBussara Sae-NgowJisnuson SvastiChulabhorn Research InstituteThe Institute of Science and Technology for Research and Development, Mahidol UniversityMahidol University2018-06-212018-06-212005-05-30Hemoglobin. Vol.29, No.2 (2005), 155-159036302692-s2.0-18844422757https://repository.li.mahidol.ac.th/handle/20.500.14594/16342Hb Kurosaki [α7(A5)Lys→Glu (AAG→GAG)], has been found for the first time in Thailand. The 30-year-old Thai male had a normal hematological profile at the steady state, but showed an abnormal hemoglobin (Hb) present at a level of 28%. Protein characterization was performed by automated sequencer analysis of the abnormal α-globin chain and amino acid analysis of the abnormal αT-1,2 peptide. Direct DNA sequence analysis of selectively amplified segments of the α1 and α2 genes showed that codon 7 of the α2-globin gene was heterozygous for AAG (Lys) and GAG (Glu). This was confirmed by restriction endonuclease digestion with Eco31I. Copyright © 2005 Taylor & Francis, Inc.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1081/HEM-200058595