Kittikhun KerdsomboonSupinda TatipSattawat KosasihChoowong AuesukareeMahidol UniversitySouth Carolina Commission on Higher Education2018-12-112019-03-142018-12-112019-03-142016-05-01Journal of Bioscience and Bioengineering. Vol.121, No.5 (2016), 543-54913474421138917232-s2.0-84961209640https://repository.li.mahidol.ac.th/handle/20.500.14594/43044© 2015 The Society for Biotechnology, Japan. Moringa oleifera leaves are a well-known source of antioxidants and traditionally used for medicinal applications. In the present study, the protective action of soluble M. oleifera leaf extract (MOLE) against cadmium toxicity was investigated in the model eukaryote Saccharomyces cerevisiae. The results showed that this extract exhibited a protective effect against oxidative stress induced by cadmium and H2O2through the reduction of intracellular reactive oxygen species. Interestingly, not only the co-exposure of soluble MOLE with cadmium but also pretreatment of this extract prior to cadmium exposure significantly reduced the cadmium uptake through an inhibition of Fet4p, a low-affinity iron(II) transporter. In addition, the supplementation of soluble MOLE significantly reduced intracellular iron accumulation in a Fet4p-independent manner. Our findings suggest the potential use of soluble extract from M. oleifera leaves as a dietary supplement for protection against cadmium accumulation and oxidative stress.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringImmunology and MicrobiologyArticleSCOPUS10.1016/j.jbiosc.2015.09.013