Bunpan T.Udomputtimekakul P.Pompimon W.Baison W.Chusiri Y.Wattananon S.Nuntasaen N.Suksen K.Chairoungdua A.Limthongkul J.Naparswad C.Pikulthong S.Athipornchai A.Mahidol University2025-05-282025-05-282025-01-01Natural Product Research (2025)14786419https://repository.li.mahidol.ac.th/handle/123456789/110395Investigation of the crude hexane and ethyl acetate extracts of the twigs and stems of D. filipes (Annonaceae) led to the discovery of five compounds: desmosdumotin D (1), a mixture of stigmasterol and β-sitosterol (2), unonal (3), isounonal (4), and cepharadione B (5). The structural elucidation of the compounds was accomplished using 1H,13C, and 2D NMR techniques. The three crude extracts of D. filipes showed weak activity versus the HIV-1 virus. The cytotoxicity assays showed that the crude hexane extract was particularly effective against A549 cell lines, with an ED50 of 15.24 mg/mL. Meanwhile, the crude ethyl acetate and methanol extracts inhibited KKU-M213 cell lines, demonstrating ED50 values of 13.42 and 10.92 mg/mL, respectively. D. filipes is a rich source of potential compounds with anti-HIV-1RT or cytotoxicity activities. This is the first report on the phytochemistry and bioactivity study of D. filipes.ChemistryBiochemistry, Genetics and Molecular BiologyAgricultural and Biological SciencesArticleSCOPUS10.1080/14786419.2025.25030162-s2.0-10500541820914786427