Roytrakul S.Jaresitthikunchai J.Charoenlappanit S.Thaisakun S.Phaonakrop N.Kittisenachai S.Supaibulwatana K.Mahidol University2025-11-212025-11-212025-12-01Scientific Reports Vol.15 No.1 (2025)https://repository.li.mahidol.ac.th/handle/123456789/113141Red beans (Phaseolus vulgaris) are gaining recognition for their potential in functional foods and nutraceuticals. This study isolated bioactive peptides under 3 kDa from red bean protein hydrolysate, generated by pepsin digestion and purified using sequential cation exchange, anion exchange, and reverse-phase chromatography. The resulting hydrolysate exhibited anticancer activity. Following chromatographic fractionation and LC-MS sequencing, eight candidate peptides were synthesized and comparatively assessed for bioactivity. Remarkably, peptide RB-8 (LIIPATSTKFL) demonstrated significant anticancer activity against colorectal (CaCO2) and cervical (SiHa) cancer cells through distinct mechanistic pathways. Furthermore, RB-7 (RGSKQRQKRQW) showed potent antihypertensive and DPPH radical scavenging activities, RB-6 (RRLRILL) displayed the strongest ABTS radical scavenging activity, and RB-1 (NLRKLKRL) exhibited the highest FRAP. These findings highlight the potential of red bean-derived peptides as natural agents for managing non-communicable diseases (NCDs) and their applicability in the food industry.MultidisciplinaryArticleSCOPUS10.1038/s41598-025-22843-02-s2.0-1050210428882045232241198802