Suganya YongkiettrakulWansadaj JaroenramNarong ArunrutWanwisa ChareanchimSupicha PannengpetchRungkarn SuebsingWansika KiatpathomchaiWichai PornthanakasemYongyuth YuthavongDarin KongkasuriyachaiThailand National Center for Genetic Engineering and BiotechnologyMahidol University2018-11-092018-11-092014-01-01Parasitology International. Vol.63, No.6 (2014), 777-78418730329138357692-s2.0-84905922588https://repository.li.mahidol.ac.th/handle/20.500.14594/34076Malaria is largely a preventable and curable disease. However, a delay or an inappropriate treatment can result in serious adverse outcomes for patient. Rapid, simple and cost-effective diagnostic tests that can be easily adapted and rapidly scaled-up at the field or community levels are needed. In this study, accelerated detection methods for the Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) dihydrofolate reductase-thymidylate synthase were developed based on the loop-mediated isothermal amplification (LAMP) method. The developed methods included the use of species-specific biotinylated primers to amplify LAMP amplicons, which were then hybridized to specific FITC-labeled DNA probes and visualized on a chromatographic lateral flow dipstick (LFD). The total LAMP-LFD assay time was approximately 1.5. h. The LAMP-LFD assays showed similar detection limit to conventional PCR assay when performed on plasmid DNA carrying the malaria dhfr-ts genes. The LAMP-LFD showed 10 folds higher detection limit than PCR when performed on genomic DNA samples from Pf and Pv parasites. The dhfr-ts LAMP-LFD assays also have the advantages of reduced assay time and easy format for interpretation of results. © 2014 Elsevier Ireland Ltd.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.parint.2014.06.004