Piamsiri SawaisornTienrat TangchaikeereeDuangporn PolpanichPanuwat MidoengRachanee UdomsangpetchAbdelhamid ElaissariKulachart JangpatarapongsaLaboratoire d'Automatique et de Génie des ProcédésMahidol UniversityPhramongkutklao College of MedicineThailand National Science and Technology Development Agency2019-08-232019-08-232018-01-01RSC Advances. Vol.8, No.26 (2018), 14393-14400204620692-s2.0-85045836662https://repository.li.mahidol.ac.th/handle/123456789/45460© 2018 The Royal Society of Chemistry. γδ T cells play a significant role in protection against cancer. Purification of γδ T cells is needed for insight when studying their anti-cancer functionality and their utilization in adoptive cell therapy. To improve the purification of γδ T cells, in this work, a composite material based on magnetic nanoparticles was developed for purification of Vγ9Vδ2 T cells, the predominant subset of γδ T lymphocytes in human peripheral blood. The epoxy-functionalized magnetic poly(divinylbenzene-co-glycidyl methacrylate) particles (mPDGs) were bio-conjugated with anti-human Vδ2 antibody to provide specific recognition sites for T cell receptors of Vγ9Vδ2 T cells. Using fluorescence-activated cell sorting (FACS) analysis, separation of Vγ9Vδ2 T cells from peripheral blood mononuclear cells of healthy donors was confirmed with high purity [89.77% (range 87.00-91.80, n = 3)]. More interestingly, the immobilized particles did not affect the viability of purified cells as high cell viability was indicated (>90%). By combining the properties of magnetic nanoparticles with specific antibodies, these immobilized particles were shown to be used as a cell-friendly purification tool of Vγ9Vδ2 T lymphocytes without any limits for the further use of cells. The purified Vγ9Vδ2 T cells using the antibody-immobilized epoxy-functionalized mPDGs could be used directly without a detachment step for further cultivation and expansion. This highlights the advantages of this method in allowing the study of cell function and further investigation of such rare T cell populations in immunotherapy.Mahidol UniversityChemical EngineeringChemistryArticleSCOPUS10.1039/c8ra01468j