Nicholas S. DabaiPornpoj PramyothinMichael F. HolickBoston University School of MedicineMahidol University2018-06-112018-06-112012-12-01Photodermatology Photoimmunology and Photomedicine. Vol.28, No.6 (2012), 307-31116000781090543832-s2.0-84868630492https://repository.li.mahidol.ac.th/handle/20.500.14594/14235Background/Purpose: Ultraviolet (UV) B irradiation may provide a safe and effective method to treat vitamin D deficiency. The objective of this study was to assess the effectiveness of a novel Sperti D/UV-Fluorescent lamp in converting 7-dehydrocholesterol (7-DHC) to previtamin D 3 in vitro and in raising serum 25-hydroxyvitamin D 3 [25(OH)D 3 ] in healthy adults. Methods: The lamp was assessed in vitro using a 7-DHC solution and a human skin sample. In a prospective cohort study, five healthy adults with skin types II and III were exposed to a 0.75 minimal erythemal dose of UV radiation over ∼9% of body surface area three times a week for 4 weeks. The main outcomes were percentage of conversion from 7-DHC to previtamin D 3 in vitro and changes in serum 25(OH)D 3 after irradiation in vivo. Results: A dose response between UV irradiation time and conversion of 7-DHC to previtamin D 3 was seen in the 7-DHC solution and surgically obtained human skin. The subjects had a significant increase in mean 25(OH)D 3 from 18.4±8.2 to 27.3±7.6ng/ml (P < 0.001) after 4 weeks of irradiation. No adverse events occurred. Conclusion: The Sperti D/UV-Fluorescent lamp is effective in converting 7-DHC to previtamin D 3 in vitro and in raising serum 25(OH)D 3 in healthy adults. © 2012 John Wiley & Sons A/S.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1111/phpp.12000