Suwatthanarak T.Ito K.Tanaka M.Sugiura K.Hoshino A.Miyamoto Y.Miyado K.Okochi M.Mahidol University2023-05-192023-05-192023-03-01Biomaterials Advances Vol.146 (2023)https://repository.li.mahidol.ac.th/handle/20.500.14594/81710As an organizer of multi-molecular membrane complexes, the tetraspanin CD9 has been implicated in a number of biological processes, including cancer metastasis, and is a candidate therapeutic target. Here, we evaluated the suppressive effects of an eight-mer CD9-binding peptide (CD9-BP) on cancer cell metastasis and its mechanisms of action. CD9-BP impaired CD9-related functions by adversely affecting the formation of tetraspanin webs—networks composed of CD9 and its partner proteins. The anti-cancer metastasis effect of CD9-BP was evidenced by the in vitro inhibition of cancer cell migration and invasion as well as exosome secretion and uptake, which are essential processes during metastasis. Finally, using a mouse model, we showed that CD9-BP reduced lung metastasis in vivo. These findings provide insight into the mechanism by which CD9-BP inhibits CD9-dependent functions and highlight its potential application as an alternative therapeutic nano-biomaterial for metastatic cancers.Chemical EngineeringArticleSCOPUS10.1016/j.bioadv.2023.2132832-s2.0-8514614696127729508