Teva PhanaksriPlearnpis LuxananilSakol PanyimWitoon TirasophonMahidol UniversityThailand National Center for Genetic Engineering and Biotechnology2018-11-232018-11-232015-10-01Journal of Bioscience and Bioengineering. Vol.120, No.4 (2015), 470-47513474421138917232-s2.0-84940898015https://repository.li.mahidol.ac.th/handle/20.500.14594/35371© 2015 The Society for Biotechnology, Japan. Strong promoter is an essential factor for production of recombinant protein in various expression systems including Bacillus subtilis. In this study, we described a strategy to improve the expression efficiency using synthetic double promoter. Assembly of the conserved elements from σB- and σA-dependent promoters constitutively improved the yield of recombinant protein approximately 2-3-fold in both exponential and stationary growth phase. The synergistic effect in the double promoter was observed only when σB-promoter was located upstream to σA-promoter but independent to its orientation. A conserved element in either -10 or -35 box of σB-promoter is sufficient to promote the synergism. Hence, this simple strategy of promoter engineering could be an effective way to generate a pool of strong constitutive promoters applicable for heterologous protein expression in B. subtilis in the future.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringImmunology and MicrobiologyArticleSCOPUS10.1016/j.jbiosc.2015.02.008