Tipparat Thiangtrongjitทิพรัตน์ เที่ยงตรงจิตต์Premjit Amornchaiเปรมจิตร อมรชัยVanaporn Wuthiekanunวรรณพร วุฒิเอกอนันต์Direk Limmathurotsakulดิเรก ลิ้มมธุรสกุลDay, Nicholas PJPiengchan Sonthayanonเพียงจันทร์ สนธยานนท์Mahidol University. Faculty of Tropical Medicine. Mahidol - Oxford Tropical Medicine Research UnitMahidol University. Faculty of Tropical Medicine. Department of Molecular Tropical Medicine and GeneticsMahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene2015-10-262021-08-302015-10-262021-08-302015-10-262013https://repository.li.mahidol.ac.th/handle/20.500.14594/63356Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.161.Melioidosis is the third most common cause of death from infectious diseases, after HIV/AIDS and tuberculosis in Northeastern Thailand. It caused by gram negative bacterium Burkholderia pseudomallei which their natural habitats are soil and surface water. Detection of B. pseudomallei in soil is important for defining geographical areas and indicating a risk of melioidosis in human and animals. Bacterial culture is conventional method for B. pseudomallei detection from soil sample. Although, it is simple but laborious and time consuming for large number of soil sample such as soil surveys or screening. Loopmediated isothermal amplification (LAMP) targeting the TSS1 gene was developed to detect B. pseudomallei in soil sample collected from Northeastern Thailand. 10 g. of soil was suspended in 10 ml TBSS-C50 broth at 40 °C for 48 hr. 250 μl of soil suspension was boiled at 100 °C for 10 min and then used for direct detection of B. pseudomallei without prior extraction. In addition, we performed methods to eliminate inhibitors in soil by adding 10 mg/ml bovine serum albumin (BSA) into the reaction. The results demonstrated that the detection of LAMP product was clearly seen when adding BSA with boiling methods.engMahidol UniversityBurkholderia pseudomalleiSoil surveyProceeding Poster