Siritida RabibhadanaSangpen ChamnongpolJanine E. TrempyNicholas P. AmbulosSkorn MongkolsukMahidol UniversityChulabhorn Research InstituteOregon State UniversityUniversity of Maryland, Baltimore County2018-08-102018-08-101993-09-30Gene. Vol.132, No.1 (1993), 113-118037811192-s2.0-0027439004https://repository.li.mahidol.ac.th/handle/20.500.14594/22532The recA gene from the bacterium Xanthomonas oryzae pv. oryzae (Xoo), a rice pathogen, was cloned based on its ability to complement DNA repair defects of Escherichia coli recA-mutants. The Xoo recA was localized to a 1.3-kb Sau3AI-XhoI fragment and, when cloned into pBR322, specifies increased methylmethanesulfonate and mitomycin C resistance to E. coli recA mutants and allows λ red-gam-to plaque on an E. coli recA-host. An E. coli recA-strain harboring a plasmid containing the Xoo recA-like gene was shown to produce a 40-kDa protein which cross-reacted with an anti-E. coli RecA antibody. A similar molecular mass protein to RecA has been detected in several Xanthomonas pathovars using an anti-E. coli RecA antibody. Furthermore, the cloned Xoo recA was shown to hybridize to genomic DNA from various Xanthomonas pathovars, but not to genomic DNA from other bacteria species under high-stringency hybridization conditions. These results indicate the isolation of the Xoo recA gene. © 1993.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1016/0378-1119(93)90522-5