Darawan WanachiwanawinRuengpung SutthentKulkanya ChokephaibulkitVanna MahakittikunJeerawan OngrotchanakunNantiya MonkongMahidol University2018-09-072018-09-072001-12-01Asian Pacific Journal of Allergy and Immunology. Vol.19, No.4 (2001), 291-2930125877X2-s2.0-0035734583https://repository.li.mahidol.ac.th/handle/20.500.14594/26549Serological evidence for Toxoplasma gondii infection in Thai pregnant women was investigated. One thousand six hundred and sixtynine blood specimens were collected from 838 HIV-seropositive and 831 HIV-seronegative pregnant women attending the antenatal-care clinic at Siriraj Hospital, Bangkok, Thailand, during a two-year period. Toxoplasma IgG antibody was detected, using a solid-phase enzyme-linked immunosorbent assay in which the membrane protein p-30 was the predominant antigen. IgG positive sera were subsequently examined for IgM antibody by the capture antibody enzyme immunoassay. The IgG antibody was found in 450 (53.7%) HIV seropositive women and 44 (5.3%) non-HIV infected women, with a statistically significant difference (p < 0.0001). Three of the 450 HIV-seropositive and 2 of the 44 HIV-seronegative sera with IgG antibody were positive for IgM antibody against T. gondii. This result suggested that HIV seropositive pregnant women had a higher risk of Toxoplasma infection with increase exposure to their offspring.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS