Sunanta RatanapoWayakorn NgamjunyapornMontri ChulavatnatolKasetsart UniversityMahidol University2018-07-042018-07-041998-12-31Plant Science. Vol.139, No.2 (1998), 141-148016894522-s2.0-0032585620https://repository.li.mahidol.ac.th/handle/123456789/18231Two new lectins, MLL 1 and MLL 2, were purified from the leaves of mulberry (Morus alba) by a procedure consisting of ammonium sulfate precipitation, affinity chromatography on a N-acetylgalactosamine-agarose column, gel filtration on a Sephacryl S-200 column, anion-exchange chromatography using a DEAE-Sephacel column and gel filtration on a Sephadex G-75 column. Both MLL 1 and MLL 2 were found to be glycoproteins with neutral sugar contents of 8.8 and 40%, respectively. They showed the same native molecular weight of 51 000. Each consisted of subunit with molecular weight of 16 500. They were highly specific for N-glycolylneuraminic acid.Mahidol UniversityAgricultural and Biological SciencesBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/S0168-9452(98)00185-X